Comparison of the inhibitory effects of resveratrol and tranilast on IgE, 48/80 and substance P dependent-mast cell activation

Results In LAD2 cells, both resveratrol and tranilast (10 ug/ml) inhibited degranulation induced by mast cell activators IgE/anti-IgE (39% and 19%, respectively; P<0.03), compound 48/80 (9% and 6%), and substance P (23% and 28%; P<0.03). This may be attributable to modulation of Ca levels, as resveratrol, and to a lesser extent tranilast, attenuated substance P-dependent increases in [Ca]i. Resveratrol and tranilast blocked cytokine formation, reducing substance P-induced TNF production (65%; P=0.04 and 46%; P=0.09, respectively), but not MCP-1 production. Furthermore, resveratrol inhibited FcepsilonRI mediated production of cysLT by 31% compared to control, whereas tranilast had no effect. The effects of resveratrol on degranulation and release of cysLT were more marked in human primary mast cells (HuMC) (64% and 90% inhibition, respectively; P<0.05), and the polyphenol was found to be significantly more efficacious than tranilast in these cells.